Composite
pT7-ECP

Part:BBa_K3647222:Design

Designed by: Natalia Savinkova, Ida Berghten, Astrid Welin, Thalia Rodriquez, Osamudiamen Iyere, Frida Haugskott, Oliver Hild Walett   Group: iGEM20_Linkoping   (2020-08-25)


pT7-Eosinophil cationic protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 109
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Added 6XHIS tag and TEV site in the N-terminus. The DNA sequence of ECP is codon-optimized for Escherichia coli. T7 promotor system with 5'UTR added upstream of the ECP gene. Downstream is a double terminator.


Source

Homo sapiens (ECP), T7 bacteriophage (transcription and translation), and Tobacco Etch virus (TEV-site).


References